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PK1010 Sigma-Aldrich

PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb

liquid, clone, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   polyclonal
biological source   rabbit
species reactivity   mouse, human
form   liquid
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C

Description

General description

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526.

Recognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.

This PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk

Application


ELISA (1:1000)

Immunoblotting (1:1000)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Packaging

50 μL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
293T cells transfected with wild-type Syk and CD8

Other Notes

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.

Tyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Protocols & Articles

Articles

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