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PMEF-CFL Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Not Treated, Passage 3

Synonym: MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells

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Properties

Related Categories Cell Culture More...
trade name   Embryomax
  Specialty Media
application(s)   Stem Cell Culture
stem cell type   Human Embryonic Stem Cells
  Mouse Embryonic Stem Cells
  PMEFs
  Induced Pluripotent Stem Cells
cell line type   Primary Mouse Embryo Fibroblasts
species   Mouse
material size   5 vials
shipped in   liquid nitrogen
storage conditions   After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Description

Other Notes

Primary Mouse Embryo Fibroblasts, Strain CF1, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.

Background information

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Physical form

The cells are provided frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3. See ES Stem Cell Manual for complete culture and medias for PMEF cells.

material package

5-6x106 ea

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

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