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PMEF-CFX Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

Synonym: MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS

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Properties

Related Categories Cell Culture More...
trade name   Embryomax
  Specialty Media
application(s)   Stem Cell Culture
stem cell type   Human Embryonic Stem Cells
  Mouse Embryonic Stem Cells
  PMEFs
  Induced Pluripotent Stem Cells
cell line type   Primary Mouse Embryo Fibroblasts
species   Mouse
material size   5 vials
shipped in   liquid nitrogen
storage conditions   Vials should be stored in the vapor phase of liquid nitrogen.

Description

Other Notes

These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.

Background information

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

material package

5-6x106 ea

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Related Content

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Many embryonic stem (ES) cell culture protocols rely on the use of a monolayer of primary mouse embryonic fibroblasts (MEF feeder cells). MEF cells perform two important roles in stem cell culture: t...
Keywords: Cell culture, Genetic, Growth factors

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