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PMEF-H Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Hygro Resistant, Strain C57/BL6, Mitomycin C Treated, Passage 3

Synonym: MEF Feeder Cells, hygro PMEFs, hygro MEFs, MEFs, Mouse Feeder Cells



Related Categories Cell Culture More...
trade name   Embryomax
  Specialty Media
application(s)   Stem Cell Culture
stem cell type   Mouse Embryonic Stem Cells
  Induced Pluripotent Stem Cells
cell line type   Primary Mouse Embryo Fibroblasts
species   Mouse
material size   5 vials
shipped in   ambient
storage conditions   After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.


Other Notes

Primary Mouse Embryo Fibroblasts, hygromycin resistant, strain C57/BL6, are Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to hygromycin.

Plating MEF Feeder Cells


1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.

Background information

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

material package

5-6x106 ea

Usage Statement

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

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Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Many embryonic stem (ES) cell culture protocols rely on the use of a monolayer of primary mouse embryonic fibroblasts (MEF feeder cells). MEF cells perform two important roles in stem cell culture: t...
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Mouse Embryonic Stem Cell Culture Procedures & Protocols

The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. Critical to this has been the ability to isolate and culture murine embryo...
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