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SCC038 Sigma-Aldrich

Human Adipose Mesenchymal Stem Cells

Human Adipose Mesenchymal Stem Cells (MSCs) are isolated from human adipose tissue and are cryopreserved at P2 to ensure optimal phenotype and the highest viability and plating efficiency.

Synonym: Human Adipose Stromal Stem Cell

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Properties

application(s)   Cell Differentiation
  Stem Cell Culture
  Cell Culture
stem cell type   Mesenchymal Stem Cells
cell line type   Mesenchymal Stem Cells
species   Human
material size   1X10⁶ cells/vial
shipped in   wet ice
storage conditions   Human Adipose Mesenchymal Stem Cells should be stored in liquid nitrogen. The cells can be passaged for at least three passages (or more than ten population double times) without significantly affect cell surface marker expression and differentiation potential.

Description

Background information

EMD Millipore’s Human Adipose MSCs are isolated from human adipose tissue and are cryopreserved at p2 to ensure optimal phenotype and the highest viability and plating efficiency. Each lot of human adipose MSCs originates from a single donor of human lipoaspirate tissue. These cells proliferate as an adherent cell monolayer and have shown a strong capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes. EMD Millipore’s Human Adipose MSCs uniformly express appropriate MSC markers including the integrin CD29, matrix receptors CD44, CD105 and stromal cell-associated markers CD73, CD90, and CD166. The cells are negative for hematopoietic lineage markers CD14, CD31, CD34, and CD45.

Components

1) 1X106 viable Human Adipose Mesenchymal Stem Cells: (Catalog No. SCC038). Store in liquid nitrogen.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

LentiBrite™ for fluorescent cell imaging for Cytoskeleton Structure

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

LentiBrite™ for fluorescent cell imaging

Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infecti...
Keywords: Autophagy, Cancer, Diseases, Flow cytometry, Fluorescent microscopy, Gene expression, Immunofluorescence, Microscopy, Tagged proteins, Transduction, Transfection

Mesenchymal Stem Cell FAQs

Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages including bo...
Keywords: Anti-inflammatory agents, Cell disruption, Clinical, Digestions, Diseases, Gene expression, Growth factors, Neurodegenerative Diseases

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook
Stem Cell Bioprocessing Group
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

Pluripotent and Multipotent Stem Cells

Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals within the body. These properties provide stem cells with unique capabi...
Keywords: AGE, Apoptosis, Cancer, Cardiovascular, Cell biology, Clinical, Diabetes, Diagnostic, Diseases, Environmental, Genetic, Genetics, Industries, Inflammation, Pharmaceutical, Stem cell biology, Transcription, Transfection

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Related Content

Mesenchymal Stem Cell Culture

Human mesenchymal stem cells (MSCs) are multipotent adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts...
Keywords: Cell culture, Cell disruption, Culture media, Diseases, Gene expression

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