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SCC109 Sigma-Aldrich

AC16 Human Cardiomyocyte Cell Line

AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.

Synonym: AC16 Ventricular Cardiomyocyte Cell Line

  •  eCl@ss 32011203

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Properties

Quality Level   100
biological source   human
application(s)   cell analysis: suitable (cell culture)
shipped in   ambient

Description

General description

AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA (1, 2). After selection in uridine-free medium to remove unfused fibroblasts, the resulting fused cells were further subcloned and subsequently screened for the presence of SV40 large T-ag, β-myosin heavy chain (βMHC) and connexin-43 (CX-43) (1).

AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag (1). The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.

References:
1. Davidson, M. M., Nesti, C., Palenzuela, L., Walker, W. F., Hernandez, E., Protas, L., Hirano, M., Isaac, N. D. (2005) Novel cell lines derived from adult human ventricular cardiomyocytes. J. Mol. Cell Cardiol. 39(1): 133-147.
2. Litzkas, P., Jha, K.K., Ozer, H.L. (1984) Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension. Mol. Cell. Biol 4(11): 2549-2552.

Cell Line Description

Cardiomyocyte Cells

Application

Research Category
Cardiac

Cardiovascular Disease

Toxicity

This product is available for sale to academic institutions or not-for-profit entities for research use. For information on commercial licensing please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

AC16 Human Cardiomyocyte Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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