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SCC150 Sigma-Aldrich

16HBE14o- Human Bronchial Epithelial Cell Line

16HBE14o- human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR.

Synonym: 16HBE, 16-HBE, 16HBEo-, 16-HBEo, 16-HBE14o

  •  eCl@ss 32011203



biological source   human
application(s)   cell analysis: suitable (cell based assays)
  cell analysis: suitable (cell culture)


General description

16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport (1). When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein (1). Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.

1. Cozens AL, et al. (1994) CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells. Am J Respir Cell Mol Biol. 10(1): 38-47.
2. Kunzelmann K, et al. (1994) cAMP-dependent activation of ion conductances in bronchial epithelial cells. Pflugers Arch 428(5-6): 590-596.
3. Koslowsky T, et al. (1994) Ca(2+)- and swelling-induced activation of ion conductances in bronchial epithelial cells. Pflugers Arch 428(5-6): 597-603.
4. Wan H, et al. (2000) Tight junction properties of the immortalized human bronchial epithelial cells Calu-3 and 16HBE14o-. Eur Respir J. 15(6): 1058-1068.
5. Illek B, et al. (2010) Sensitivity of chloride efflux vs. transepithelial measurements in mixed CF and normal airway epithelial cell populations. Cell Physiol Biochem 26(6): 983-990.

Cell Line Description

Epithelial Cells


This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.


• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases

Figure 1. The 16HBE14o- cell line is a suitable model for studying molecular mechanisms that retains characteristic features of normal differentiated bronchial epithelial cells.
Keywords: Autophagy, Cancer, Cell culture, Chromatography, Culture media, Diseases, Gas chromatography, Gene expression, Inflammation, Mass spectrometry, Metabolism, Methylations, Respiratory, Ubiquitinations

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