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SCM018 Sigma-Aldrich

Embryoid Body (EB) Formation Medium

The Embryoid Body Formation Medium (EB Media) has been optimized & qualified to support the formation of embryoid body.

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Properties

Related Categories Cell Biology, Cell Culture, Classical Media and Salts, Stem Cell Biology, Stem Cell Media More...
application(s)   Cell Differentiation
stem cell type   Mouse Embryonic Stem Cells
  Induced Pluripotent Stem Cells
media form   Liquid
material size   100 mL
shipped in   dry ice
storage conditions   Stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.

Description

Other Notes

The Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.

Background information

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.

Physical form

Embryoid Body (EB) Formation Medium is a proprietary formulation that contains fetal bovine serum.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Poster T-3056: Efficient Differentiation Of Human Pluripotent Stem Cell To Branching Lung Bud Organoids & Maturation Into Alveolar Lung Organoids.
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Protein assay, Vitamins

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