• USA Home
  • SCM045 - Human Mesenchymal-XF Expansion Medium

EMAIL THIS PAGE TO A FRIEND
SCM045 Sigma-Aldrich

Human Mesenchymal-XF Expansion Medium

Xeno-free mesenchymal stem cell media specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues.

Purchase

Properties

Related Categories Cell Biology, Stem Cell Biology, Stem Cell Media More...
trade name   Chemicon
application(s)   Cell Culture
  Stem Cell Culture
stem cell type   Mesenchymal Stem Cells
media form   Liquid
material size   500 mL
shipped in   dry ice
storage conditions   Frozen medium should be stored at -20ºC until expiration date.
Thawed medium can be stored at 2 to 8ºC for up to 1 month.
Multiple freeze/thaw cycles are not recommended

Description

Background information

The Human Mesenchymal-XF Expansion Medium is a xeno-free medium specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues. The medium contains 2% human serum, is free of animal-derived components and supports the growth of human MSCs at rates equal to or greater than other commercial xeno-free/serum-free media and media supplemented with 2-10% FBS. Human MSCs cultured in the medium retain self-renewal and multi-lineage differenitation potential. The Human Mesenchymal-XF Expansion Medium does not contain any antimicrobials, phenol red, or any other components that can cause cell stress and “masking effects” that may influence experimental results.

Components

All components used within the media are of human origin and do not contain any animal derived components.

Physical form

The Human Mesenchymal-XF Expansion Medium is a complete medium and contains all the necessary growth factors and supplements to support human mesenchymal stem cell growth.

Application

A separate ECM solution is not required to facilitate cell attachment using the human mesenchymal-XF expansion medium.

Quality Assurance

These products are manufactured with the highest quality of raw materials, with exacting standards and production procedures to ensure lot-to-lot consistency. Every lot of medium is extensively tested for the following parameters:

Sterility Testing: Negative for bacterial and fungal growth
pH: 7.5 ± 0.3
Cell Testing: Rate of proliferation and morphology
Osmolality: 255 ± 15 mOsm
Endotoxin: < 0.5 EU/mL

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Mesenchymal Stem Cell FAQs

Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages including bo...
Keywords: Anti-inflammatory agents, Cell disruption, Clinical, Digestions, Diseases, Gene expression, Growth factors, Neurodegenerative Diseases

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook
Stem Cell Bioprocessing Group
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

Pluripotent and Multipotent Stem Cells

Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals within the body. These properties provide stem cells with unique capabi...
Keywords: AGE, Apoptosis, Cancer, Cardiovascular, Cell biology, Clinical, Diabetes, Diagnostic, Diseases, Environmental, Genetic, Genetics, Industries, Inflammation, Pharmaceutical, Stem cell biology, Transcription, Transfection

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Related Content

Mesenchymal Stem Cell Culture

Human mesenchymal stem cells (MSCs) are multipotent adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts...
Keywords: Cell culture, Cell disruption, Culture media, Diseases, Gene expression

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?