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SCR127 Sigma-Aldrich

Synthetic Laminin Peptide for Rat Neural Stem Cells

  •  eCl@ss 32160405

  •  NACRES NA.75

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Properties

Quality Level   100
assay   >95% (HPLC)
form   lyophilized
application(s)   cell culture | mammalian: suitable
input   sample type neural stem cell(s)
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Lamb2(25473)

Description

General description

Laminin, an extracellular matrix protein of the cell basement membrane, is involved in various biological activities including cell adhesion, proliferation, migration, invasion, and differentiation These heterotrimeric macromolecules - consisting of α, β, and γ chains - are difficult to extract and purify in consistently active quantities from native tissues. While some of these laminin isoforms can be isolated from either cultured cells or placental tissues, in both cases, overall protein yields tend to be relatively low and suffer from batch to batch variations. Therefore, for many stem cell scientists, it has become necessary to test each new lot laminin to ensure that the activity level of the protein was sufficient to sustain their stem cell culture.

To overcome this time barrier, a defined synthetic laminin substrate with similar binding and functional properties of the native laminin protein is considered to be highly advantageous as it would eliminate the requirement to test and screen new lots of native protein. Millipore’s Synthetic Laminin Peptide is a suitable replacement for the native laminin protein, and has been optimized to specifically support cell adhesion, proliferation and multi-lineage differentiation of rat neural stem cells (NSCs) in vitro. Rat neural stem cells grown on tissue culture plates coated with the synthetic laminin peptides display the characteristic neural stem cell markers, Nestin and Sox-2, and furthermore possess the capacity to preferentially differentiate down both glial and neural lineages. Millipore’s Synthetic Laminin Peptide is an inexpensive, reproducible, and defined ECM substrate alternative for the in vitro culture of rat neural stem cells.

(Note: This product has been optimized to support the growth of rat NSCs, and is not recommended for use with human or mouse NSCs).

Packaging

3×5 mg in 5 x 3 mg

5 x 3 mg

Physical form

Lyophilized peptide

Storage and Stability

The Synthetic Laminin Peptide (Catalog No. SCR127) is shipped lyophilized and should be stored at -20°C upon receipt. Once the peptide has been reconstituted, it should be stored at 4ºC and used within 1 month.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Neural Stem Cell FAQs

The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of re...
Keywords: Cell culture, Central Nervous System, Centrifugation, Clinical, Diffusion, Digestions, Diseases, Gene expression, Immunocytochemistry, Neurodegenerative Diseases, Parkinson Disease

Protocols

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

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