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  • SCT033 - BioTracker 520 Green Hypoxia Dye

SCT033 Sigma-Aldrich

BioTracker 520 Green Hypoxia Dye

Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels.

Synonym: Live cell imaging probe

  •  eCl@ss 32161000



application(s)   cell analysis: suitable (cell based assay)
  cell analysis: suitable (cell based assays)
detection method   fluorescent
shipped in   ambient


General description

Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells. Hypoxia in living tissue closely relates to a wide array of pathologies, including cancer and ischemia. Existing hypoxia detection probes, such as pimonidazole, require cell fixation followed by immunostaining.

The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.

Spectral Properties
Absorbance: 498nm
Emission: 520nm


Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Physical form


Storage and Stability

Store BioTracker 520 Green Hypoxia Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Hypoxia Detection Assays

Defined as an oxygen deficiency in tissues, hypoxia is a hallmark of primary tumors that occurs due to impaired vascularization and the increased respiratory demand of rapidly proliferating cancer ce...
Keywords: Apoptosis, Buffers, Cancer, Cell culture, Cell proliferation, Chromatin immunoprecipitation, Cytotoxins, Environmental, Enzyme-linked immunosorbent assay, Enzymology, Flow cytometry, Gene expression, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunostaining, Individual protein Immunoprecipitation, Microarray Analysis, Peptide synthesis, Respiratory, Transcription, Western blot

Related Content

Live Cell imaging of Hypoxia [VIDEO]

A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions ...

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