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SCT045 Sigma-Aldrich

BioTracker ATP-Red Live Cell Dye

Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health.

Synonym: Live cell imaging probe

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Properties

Related Categories Cell Culture, Reagents and Supplements More...
detection method   Fluorescence
application(s)   Cell Based Assays
material size   1 mg
shipped in   ambient
storage conditions   Store BioTracker ATP-Red Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Description

Other Notes

Adenosine triphosphate (ATP) is the primary energy source for all cellular processes. ATP also functions as a signaling molecule for regulating cell movement, neurotransmission and ion channel functions. ATP is localized in mitochondria, where cellular respiration occurs. ATP levels can be used to measure cell proliferation and cell cycle dynamics.

The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.

Spectral Properties
Absorbance: 510nm
Emission: 570nm

References
Chang YT et al. A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6.

Physical form

Lyophilized

Application

Live cell fluorescent imaging

Quality Assurance

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 561.48 g/mol

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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