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SNAP2MIDI Millipore

SNAP i.d. 2.0 Protein Detection System-Midi (8.5 x 13.5 cm)

Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..

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Properties

trade name   Snap i.d.
detection method   Chemiluminescence
  Fluorescence
  Chromogenic
compatible blocking solutions   bløk®-CH Buffer (cat. no. WBAVDCH01)
  bløk®-FL Buffer (cat. no. WBAVDFL01)
  bløk®-PO Buffer (cat. no. WBAVDP001)
  Commercially available blocking reagents
compatible detection reagents   Luminata Western HRP Substrates
  Commercially available detection reagents
compatible membranes   PVDF (Immobilon membranes)
  Nitrocellulose
application(s)   Western Blotting
material size   1/pk
shipped in   ambient
storage temp.   Room Temperature

Description

Other Notes

This system includes the SNAP i.d. 2.0™ Base, two Midi Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start Guide

materials required but not delivered

Vacuum Pump (WP62 or WP61)

Application

For use on immunoblots.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

SNAP i.d.® 2.0 Protein Detection System for Western Blotting

The immunodetection phase of Western blotting, consisting of blocking, antibody incubations, and washing steps, may be subject to experimental variability. The SNAP i.d. 2.0 system both accelerates i...
Keywords: Buffers, Diagnostic, Western blot

Protocols

Recipe Calculators for Western Blotting Buffers & Solutions

The recipes listed enable the accurate preparation of commonly used solutions for Western blotting, whether you are mixing low volumes for a few experiments or multiple liters for the entire lab to u...
Keywords: Buffers, Detergents, Western blot

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