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  • ST1049 - Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

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ST1049 Sigma-Aldrich

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

liquid, clone MCP231, Calbiochem®

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Properties

Related Categories 1-A0, Alphabetical Index, Antibodies, Primary Antibodies
clone   MCP231, monoclonal
biological source   mouse
species reactivity   yeast, human, rabbit, mouse, rat
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.

Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

Immunogen

Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)

dinitrophenylated proteasomes

Application


Immunoblotting (1:1000, chemiluminescence)

Immunoprecipitation (not recommended)

ELISA (see comments)

Immunofluorescence (see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Frozen Sections (see application references)

Packaging

100 μL in Plastic ampoule

Warning

Toxicity: Highly Toxic (H)

Analysis Note

Positive Control
Human placental tissue

Other Notes

Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.

Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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