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ST1553 Sigma-Aldrich

Anti-VCP Mouse mAb (4A8)

liquid, clone 4A8, Calbiochem®

Synonym: Anti-Valosin-Containing Protein

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, V
clone   4A8, monoclonal
biological source   mouse
species reactivity   human
form   liquid
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.

Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.

This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.

Immunogen

Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein

Application



ELISA (see comments)

Immunoblotting (1-5 µg/ml)

Paraffin Sections (3 µg/ml)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)

Analysis Note

Positive Control
HePG2 cells, human placenta tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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