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  • ST1704 - Anti-TOMM22 Mouse mAb (4G4)

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ST1704 Sigma-Aldrich

Anti-TOMM22 Mouse mAb (4G4)

liquid, clone 4G4, Calbiochem®

Synonym: Anti-Translocase of Outer Mitochondrial Membrane 22

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TN-TP
clone   4G4, monoclonal
biological source   mouse
species reactivity   rat, human, mouse
form   liquid
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.

Recognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.

This Anti-TOMM22 Mouse mAb (4G4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM22.

Immunogen

Full-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein

Application





ELISA (see coments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

Paraffin Sections (3 µg/ml)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)

Analysis Note

Negative Control
293T cells

Positive Control
PC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

WGK Germany 
nwg
Protocols & Articles

Articles

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