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TMS-009 Sigma-Aldrich

Safranin-O Staining Solution

Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells (MSCs).

Synonym: Basic Red 2

  •  NACRES NA.75

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains, Nucleic Acid Stains, Nucleic Acid Stains For Microscopy More...
Quality Level   100
concentration   0.1 % (w/v) in distilled water
application(s)   cell analysis: suitable (cell based assays)
  cell analysis: suitable (cell differentiation)
  cell culture | stem cell: suitable

Description

General description

Safranin-O, also known as basic red 2, is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red. It can also be used for the detection of cartilage, mucin and mast cell granules. Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research

Research Sub Category
Osteobiology

Arthritis

Quality

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Physical form

Product is presented as a 0.1% w/v solution diluted in distilled water.

Storage and Stability

Store at room temperature for up to 4 months from date of receipt. If Safraninin-O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Mesenchymal Stem Cell FAQs

Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages including bo...
Keywords: Anti-inflammatory agents, Cell disruption, Clinical, Digestions, Diseases, Gene expression, Growth factors, Neurodegenerative Diseases

Protocols

Mesenchymal Stem Cell Culture Protocols | MSC Culture Media

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Related Content

Mesenchymal Stem Cell Culture

Human mesenchymal stem cells (MSCs) are multipotent adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts...
Keywords: Cell culture, Cell disruption, Culture media, Diseases, Gene expression

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