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WBKLS Millipore

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

  •  eCl@ss 42029053

  •  NACRES NB.22



Related Categories Membranes & Blotting Paper, Millipore PVDF Membranes for Western Blotting, Molecular Biology, Protein Electrophoresis, Proteomics,
Quality Level   400
packaging   pkg of 250 mL Luminol
  pkg of 250 mL Peroxide solution
mfr. no.   Immobilon®
application(s)   western blot: suitable
membrane area   5000  cm2 , membrane coverage
conjugate   peroxidase conjugate
detection method   chemiluminescent
shipped in   wet ice
storage conditions   2 to 8 °C



For use on immunoblots.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Dot/Slot (Filtration) Blotting: Western Blot Troubleshooting

Dot blotting is an ideal technique for quickly assessing the levels of a target antigen across many samples at once. Also, it is a popular method for epitope mapping and screening antibodies for targ...
Keywords: Detergents, Dot blot, Filtration

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

Figure 1. Structure of glycosylated human glutaminyl cyclase (PDB 3SI0), NGL Viewer (AS Rose et al. (2018) NGL viewer: web-based molecular graphics for large complexes. Bioinformatics doi:10.1093/bio...
Keywords: Buffers, Catalysis, Cell disruption, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Glycosylations, Homogenization, N-linked glycosylation, O-linked glycosylation, PAGE, Phosphorylations, Sample preparations, Separation, Ubiquitinations, Western blot


Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

Peer-Reviewed Papers


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