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WBULS0100 Millipore

Immobilon ECL Ultra Western HRP Substrate

Western blotting, HRP

  •  eCl@ss 42029053

  •  NACRES NB.22



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Quality Level   300
application(s)   western blot: suitable
detection method   chemiluminescent
shipped in   ambient


General description

Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.


Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.

Sufficient for 1000 cm²


Solution A- 50 mL

Solution B- 50 mL

Storage and Stability

Store at 2–8 °C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Immunodetection: Western Blot Troubleshooting

Perhaps more than any other step in Western blotting, immunodetection conditions are subject to the greatest number of variables, in part because this step depends on antibody:antigen recognition. Ou...
Keywords: Amplification, Cell disruption, Degradations, Detergents, Gene expression, Glycosylations, Immunohistochemistry, Phosphorylations, Ubiquitinations, Western blot

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

Figure 1. Structure of glycosylated human glutaminyl cyclase (PDB 3SI0), NGL Viewer (AS Rose et al. (2018) NGL viewer: web-based molecular graphics for large complexes. Bioinformatics doi:10.1093/bio...
Keywords: Buffers, Catalysis, Cell disruption, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Glycosylations, Homogenization, N-linked glycosylation, O-linked glycosylation, PAGE, Phosphorylations, Sample preparations, Separation, Ubiquitinations, Western blot

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