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04683633001 Roche

Universal ProbeLibrary Set, Human

(The Universal ProbeLibrary will be phased out by the end of 2020 and stock will be available until it is depleted. The Assay Design Center will be available through December 31st, 2020)



Quality Level   100
mfr. no.   Roche
packaging   set of of 90 UPL probes, 125 μl, 10 μM, each


General description

Universal ProbeLibrary Set Human; Instructions For Use

Set of 90 short hydrolysis probes for gene expression quantification of human genes by real-time PCR.

The Universal ProbeLibrary Set, Human contains probes #1 to #90 of the 165 Universal ProbeLibrary Probes. In combination with the unique ProbeFinder assay design software, these 90 hydrolysis probes provide real-time PCR assays for more than 98% of the 37,000 human gene transcripts included in the NCBI Reference Sequence Database.
The probes are dual-labeled with a reporter fluorophore (FAM) and a dark quencher dye. They are used in standard real-time PCR assays, on standard real-time PCR instrumentation.


Use the Universal ProbeLibrary Set, Human to obtain rapid and flexible quantification of gene expression levels for virtually all human genes included in the NCBI Reference Sequence Database, in real-time PCR assays on the LightCycler® 480 System, the LightCycler® Carousel-Based System, or other commercially available real-time PCR instruments. Perform an assay with a probe selected from the Universal ProbeLibrary Set, Human by following established real-time PCR protocols using the LightCycler® 480 Probes Master or the LightCycler® TaqMan® Master on the LightCycler® Carousel-Based System, or the FastStart TaqMan® Probe Master (or other real-time PCR-compatible reagents) on other real-time PCR instruments.
Easily design gene-specific expression-quantification assays by using the free web-based ProbeFinder software at the Assay Design Center, accessible at www.universalprobelibrary.com.

Features and Benefits

• Significantly reduce assay design time and time to result.
• Combine high assay specificity with flexibility and convenience.
• No special hardware or unique reaction conditions are required.


90 vials, each containing 125 μl, 10 μM of a Universal ProbeLibrary probe, 5′-end labeled with fluorescein (FAM) and 3′-end labeled with a dark quencher dye.


All probes in the Universal ProbeLibrary Set, Human have passed a real-time PCR performance test using the matching primers and control templates from the Universal ProbeLibrary Control Set. Each probe is analyzed by anion-exchange HPLC and MALDI-MS to ensure purity and quality.

Other Notes

LightCycler® 480 Real-Time PCR System: Innovative Solutions for Relative Quantification Article (PDF, 1.28 MB)

Universal ProbeLibrary System Performance Data

Universal ProbeLibrary System Technology

Universal ProbeLibrary System User Statements and Application

Millions of qPCR assays readily available using pretested probes Article (PDF, 715.57 KB)

qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway Article (PDF, 131.63 KB)

Step-by-Step Workflow for Small Sample Quantitative Real-Time PCR Confirmation of Microarray Results Article (PDF, 873.32 KB)

For life science research only. Not for use in diagnostic procedures.

Legal Information

LightCycler is a registered trademark of Roche

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles
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