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04743784001 Roche

GC-RICH PCR System, dNTPack

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Properties

usage   sufficient for ≤50 reactions
packaging   pkg of 100 U
mfr. no.   Roche
parameter   72 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C (−15°C to −25°C)

Description

General description

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

Application

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.

Features and Benefits

• Ease access to difficult templates:-
including GC-rich targets and repetitive sequences.
• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.
• Cost-effective:-
use the convenient premixed solution of PCR grade NTPs.

Packaging

1 kit containing 6 components

Quality

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.

Unit Definition

Volume Activity: 2 U/μl

Preparation Note

Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit component only

Description

Product #

Add to Cart

GC-RICH Enzyme Mix, in storage buffer    
GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl2 and DMSO 5x concentrated    
GC-RICH Resolution Solution 5 M    
MgCl2 Stock Solution 25 mM    
Water, PCR Grade    
PCR Nucleotide Mix    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

GC-RICH PCR System, dNTPack Troubleshooting

TROUBLESHOOTING GC-RICH TEMPLATES Template quality significantly influences PCR outcome. For the amplification of GC-rich targets up to 5 kb in length, DNA with repetitive sequences, and DNA mixtures...
Keywords: Amplification, Detergents, Gas chromatography, Polymerase chain reaction, Titrations

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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