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04869877001 Roche

Universal ProbeLibrary Extension Set

(The Universal ProbeLibrary will be phased out by the end of 2020 and stock will be available until it is depleted. The Assay Design Center will be available through December 31st, 2020)

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Properties

Quality Level   100
mfr. no.   Roche
packaging   set of of 75 UPL Probes, 125 μl, 10 μM, each
of 75 UPL probes, 125 μl, 10 μM, each.

Description

General description

Universal ProbeLibrary Extension Set, Probes #91 - #165; Instructions For Use

Set of 75 short hydrolysis probes for gene expression quantification of multiple organism levels by real-time PCR.

Application

Use the Universal ProbeLibrary Extension Set in combination with the Universal ProbeLibrary Set, Human to obtain a complete set of all 165 Universal ProbeLibrary Probes. This combination of sets enables the quantification of transcripts of all organisms included on the online Universal ProbeLibrary Assay Design Center. In addition, this combination of sets is utilized when designing assays for organisms not included on the Universal ProbeLibrary Assay Design Center by selecting the "Other Organism" link.

Features and Benefits

The set contains Universal ProbeLibrary Probes #91 to #165. The probes are dual-labeled with a reporter fluorophore (FAM) and a dark quencher dye. They are used in standard real-time PCR assays on standard real-time PCR instrumentation.

• Significantly reduce assay design time and time to result.
• Combine high assay specificity with flexibility and convenience.
• No special hardware or unique reaction conditions are required.

Components

10 μM solution of a Universal ProbeLibrary probe, 5′ labeled with fluorescein (FAM) and 3′ labeled with a dark quencher dye.

Quality

All probes in the Universal ProbeLibrary Extension Set have passed a real-time PCR performance test using the matching primers and control templates from the Universal ProbeLibrary Control Set.
Each probe is analyzed by anion-exchange HPLC and MALDI-MS to ensure purity and quality.

Other Notes

Universal ProbeLibrary System Performance Data

Universal ProbeLibrary System Technology

Universal ProbeLibrary System User Statements and Application

Entering Multiple Sequences Article (PDF, 870.62 KB)

Millions of qPCR assays readily available using pretested probes (PDF, 715.57 KB)

qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway Article (PDF, 131.63 KB)

Universal ProbeLibrary Set: One Transcriptome - One Kit Article (PDF, 278.17 KB)

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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