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10102105001 Roche

Adenosine Deaminase (ADA)

from calf intestine

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
Quality Level   100
form   solution
specific activity   ~200 units/mg protein (At 25 °C with adenosine as the substrate.)
mol wt   230-440 kDa (high)
  30-47 kDa (low )
packaging   pkg of 2 mL (10 mg)
mfr. no.   Roche
optimum pH   7.0-7.4
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.

Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.

Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Biochem/physiol Actions

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Preparation Note

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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