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10102547001 Roche

AMP-PNP

Adenylyl-imidodiphosphate

Synonym: β,γ-Imidoadenosine 5′-triphosphate lithium salt hydrate, AMP-PNP, ATP[β,γ-NH], Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate, Adenylyl imidodiphosphate lithium salt hydrate, App(NH)p

  • CAS Number 25612-73-1 (free acid)

  • Empirical Formula (Hill Notation) C10H17N6O12P3 · xLi+ · yH2O

  • Molecular Weight 506.20 (free acid basis)

  •  Beilstein/REAXYS Number 6047109

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Phosphate Donors
Quality Level   100
form   powder
mol wt   Mr 506.2 (ANP-PNP)
  Mr 529.9 (AMP-PNP-Li4)
packaging   pkg of 25 mg
mfr. no.   Roche
shipped in   dry ice
SMILES string   [Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O

Description

General description

AMP-PNP (adenylyl-imidodiphosphate) is a non-hydrolysable analogue of ATP. It inhibits fast axonal transport and facilitates the interaction between membranous organelles and microtubules.

AMP-PNP(adenylyl-imidodiphosphate) is an artificial substrate of enzyme, adenylate cyclase. Adenylate cyclase helps to hydrolyze adenylyl-imidodiphosphate slowly than ATP.

Application

AMP-PNP has been used in the preparation of nucleotide solutions, two-motor fluorescence assay, preparation of flow-cells with kinesin and cascade binding assays.

AMP-PNP is a competitive inhibitor of most ATP-dependent systems. A good substrate for enzymes hydrolyzing between the α- and β-phosphorus atom, yet is resistant to enzymes cleaving between the β- and γ-phosphorus atom. Substrate of snake venom phosphodiesterase and adenylate cyclase.
AMP-PNP has been used for two-motor fluorescence assay.

Features and Benefits

Contents
88% AMP-PNP (from N), 4.5% lithium, 6% water

Quality

Contaminants: ≤1.5% Pi , <0.1% ATP (enzymatically)

Storage and Stability

Store at -15–-25 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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