10102946001 Roche


Synonym: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2′-azino-di-[3-ethylbenzthiazoline sulfonate (6)], AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), azino-di-[3-ethylbenzthiazoline sulfonate (6)], 2,2′-

  • CAS Number 30931-67-0

  • Empirical Formula (Hill Notation) C18H24N6O6S4

  • Molecular Weight 548.68

  •  Beilstein/REAXYS Number 7329461

  •  MDL number MFCD00010404

  •  PubChem Substance ID 329748893



Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Peroxidase Substrates, Substrates by Enzyme More...
Quality Level   100
assay   >98%
mol wt   548.68
packaging   pkg of 2 g
mfr. no.   Roche
solubility   50 mg/mL
λmax   414 nm
shipped in   wet ice


General description

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.


ABTS (C18H16N4O6S4-(NH4)2) is a peroxidase substrate for ELISA.

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.


Purity: >98% (absorbance), chromatographically homogeneous

Preparation Note

Working concentration: ABTS should be dissolved as follows:

100 mg ABTS in in 100ml 3.25 mM sodium perborate, 39.8 mM citric acid, 60 mM disodium hydrogen phosphate, pH 4.4-4.5 (see also PI of SA-peroxidase). This buffer corresponds to the single reagent ABTS-Buffer, Cat. No. 1204530. The formed product is green and soluble in water. Measurement at 405 nm.

Dilution of ABTS:

ABTS/ buffer solution (9.1 mM ABTS; pH 5.0): 99,9 mg ABTS in 20 ml potassium phosphate buffer (0.1M; pH 5.0).

Storage and Stability

Store at 2 to 8 °C. (It is also possible to store at -15 to -25 °C)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ABTS is a trademark of Roche

Safety & Documentation

Safety Information

NONH for all modes of transport


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