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10107735001 Roche

NADH

Grade I, disodium salt

Synonym: β-DPNH, β-NADH, β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, DPNH, Diphosphopyridine nucleotide, reduced form, NADH

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
InChI Key   FWBNCIFELNNJCX-UHOVGGJYSA-L
assay   100% (dry weight)
form   amorphous powder
mol wt   Mr 665.4 (NADH)
  Mr 709.4 (NADH-Na2)
packaging   pkg of 500 mg
mfr. no.   Roche
pH   7.5 (100 mg/mL in water, ±0.5)
  7.5 ± 0.5 (100 mg/mL in water)
absorption   Amax 260 and 340 nm
shipped in   wet ice
storage temp.   2-8°C

Description

Application

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Nicotinamide adenine dinucleotide (NADH) has been used in:
• nicotinamide adenine dinucleotide (NAD+) /NADH cycling assay.
• adenosine triphosphate (ATP)ase assay.
• pyruvate kinase enzyme assay.

Biochem/physiol Actions

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Packaging

Packaged by solid weight.

Quality

Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard)
Organic solvents: <4% (GC)

Preparation Note

Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C.

Analysis Note

Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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