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10107921001 Roche

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

Synonym: s7 nuclease

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Properties

Quality Level   100
biological source   Staphylococcus aureus
form   lyophilized
specific activity   ~15,000 U/mg
packaging   pkg of 15,000 U
mfr. no.   Roche
optimum pH   7.5
shipped in   wet ice

Description

Application

• Removal of endogenous RNA from in vitro translation systems
• RNA sequencing experiments

Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

Biochem/physiol Actions

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA.<REFERENCE ID=""540296"" TYPE=""literature""/> Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

Unit Definition

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.

Storage and Stability

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

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