EMAIL THIS PAGE TO A FRIEND
10128023001 Roche

NADH

Grade II, disodium salt

Synonym: β-DPNH, β-NADH, β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, DPNH, Diphosphopyridine nucleotide, reduced form, NADH

Purchase

Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
InChI Key   FWBNCIFELNNJCX-UHOVGGJYSA-L
assay   98%
mol wt   (NADH-Na2: Mr = 709.4
NADH: Mr = 665.4)
packaging   pkg of 1 g
mfr. no.   Roche
storage condition   protect from light
absorption   Amax 260 and 340 nm
shipped in   wet ice

Description

Application

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.

NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

Biochem/physiol Actions

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Packaging

Packaged by solid weight.

Quality

Purity: 98% β-NADH-Na2 based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260 nm-A340 nm: <2.40
Organic solvents: <4% (GC)

Specifications

Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at 334 nm (Hg), 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at 365 nm (Hg)

Formula:
C21H27N7O14P2Na2

pH value in water: 7.5 ± 0.5 (100 mg/ml)

Physical form

Amorphous powder

Preparation Note

Activator: Mg2+ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?