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10411523001 Roche

Luciferase

from Photinus pyralis (American firefly)

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Properties

Related Categories Cell Biology, General Metabolic Enzymes, General Metabolic Enzymes I-Z, Metabolomics
form   lyophilized (recrystallized twice)
specific activity activity   ~8 units/g protein (At 25 °C with 0.1 mM luciferin and 0.7 mM ATP as the substrates (0.1 M Tris-acetate, pH 7.75).)
packaging   pkg of 1 mg
mfr. no.   Roche
optimum pH   7.5
shipped in   wet ice

Description

General description

Luciferase is a class of enzymes naturally found in organisms like protists, fungi, insects, bacteria which belong to phylogenic kingdom. It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light.

Application

Determination of ATP in ATP-generating or ATP-consuming reactions. Determination of the activity of enzymes such as creatine kinase and ATPases. Under optimal conditions, 1 photon is released per molecule ATP in the bioluminescence assay.
One mg is sufficient for approximately 5,000 ATP determinations in the concentration range of 0.1 nM to 1 μM.

Quality

Contaminants: <1 mU ATPase/mg lyophilizate, and <10 mU nucleoside diphosphatase kinase/mg lyophilizate. The lyophilizate contains neither luciferin nor arsenate.

Sequence

Enzyme consists of two subunits, one of them is active.

Preparation Note

Storage conditions (working solution): -15 to -25 °C
Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Information Note: Do not store in plastic vessels.

Reconstitution

Cool the original glass vial (1 mg or 5 mg protein, respectively) to 0 °C in an ice-bath and dissolve the containing luciferase by adding 1 ml or 5 ml, respectively, 0.5 M ice-cold Tris-acetate buffer, pH 7.5, and let it stand for 10 minutes. (The dissolved enzyme is sensitive to shaking and to storage in plastic vessels). This concentrated solution is stable for several
months when stored frozen in portions of approximately 50 μl. Diluted solutions are unstable and should be prepared directly before use and should be kept in an ice-bath.
One microgram luciferase corresponds to approximately 107 arbitrary luciferase units.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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