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10634425001 Roche

n-Octylglucoside

non-ionic

Synonym: n-Octyl glucoside, OGP, Octyl β-D-glucopyranoside

  • CAS Number 29836-26-8

  • Empirical Formula (Hill Notation) C14H28O6

  • Molecular Weight 292.37

  •  Beilstein/REAXYS Number 84118

  •  MDL number MFCD00063288

  •  PubChem Substance ID 329748920

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Properties

Related Categories Biochemicals and Reagents, Detergents, Detergents by Applications, Membrane Protein Solubilization
Quality Level   100
InChI Key   HEGSGKPQLMEBJL-RKQHYHRCSA-N
assay   99% (GC)
mol wt   micellar avg mol wt 25,000
  292.4
packaging   pkg of 10 g
mfr. no.   Roche
aggregation number   84
application(s)   dialysis: suitable
CMC   20-25 mM (20-25°C)
transition temp   cloud point >100 °C
shipped in   ambient

Description

General description

1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

Application

N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.

Non-ionic, dialyzable detergent for the solubilization and isolation of membrane proteins. Has been shown to increase the resolution of proteins in 2D gels.

Quality

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6

Preparation Note

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.

Storage and Stability

Store at 15 to 25 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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