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10663573001 Roche

BM-Condimed

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Properties

Related Categories Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements,
form   solution
packaging   pkg of 100 mL
mfr. no.   Roche
shipped in   wet ice

Description

General description

BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.

Application

BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.

Physical form

Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate.

Preparation Note

Working concentration: 1 to 100μg/ml
1 to 100μg/ml
Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.

Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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