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10789704001 Sigma-Aldrich

Triton X-100

non-ionic, viscous liquid

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether, Triton X-100

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Properties

Related Categories Biochemicals and Reagents, Detergents, Detergents by Applications, Membrane Protein Solubilization
form   viscous liquid
mol wt   micellar avg mol wt 80,000
  average mol wt 625
packaging   pkg of 100 mL
mfr. no.   Roche
storage condition   protect from light
aggregation number   100-155
application(s)   electron microscopy: suitable
  immunofluorescence: suitable
CMC   0.2-0.9 mM (20-25°C)
transition temp   cloud point 65 °C
  pour point ~7 °C
HLB   13.5
shipped in   wet ice
SMILES string   CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI   1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key   HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Octophenolpoly(ethyleneglycolether)n
Especially used for purification in membrane research
Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.

Application

• Triton X-100 is one of the most commonly used non-ionic detergents for solubilizing membrane during isolation of membrane-protein complexes
• Used for native gel electrophoresis
It has been used in several types of buffer solution such as Embryo wash solution, RIPA buffer, NSucrose buffer, and Nuclear extraction buffer (NEB).

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Preparation Note

Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM

Storage and Stability

Store at 2 to 8 °C. (Keep under N2!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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