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10901393001 Roche

Glycogen

from mussels

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Properties

Quality Level   100
form   solution
packaging   pkg of 1 mL (20 mg)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Application

This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.

Features and Benefits

Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.

Contents
Aqueous solution, 20 mg/ml

Quality

Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Glycogen Protocol & Troubleshooting

Recovery of RNA: For quantitative recovery of low concentrations of RNA (ng/ml), an inert coprecipitant (e.g., glycogen, yeast RNA, or linear acrylamide) should be used.

Peer-Reviewed Papers
15

References

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