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10901393001 Roche

Glycogen

from mussels

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Properties

form   solution
packaging   pkg of 1 mL (20 mg)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.

Contents
Aqueous solution, 20 mg/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.

Application

This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


LC-MS Grade Solvents and Reagents

Same Products - New Packaging
Protocols & Articles

Protocols

Analyzing real-time PCR data by the comparative CT method

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PC...
Keywords: Amplification, Anti-inflammatory agents, Apoptosis, Cancer, Clinical, Dehydration reaction, Diagnostic, Gene expression, Hormones, Methylations, Microarray Analysis, Molecular biology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription, Transduction, Transfection, cDNA microarrays, transformation

Glycogen Protocol & Troubleshooting

Recovery of RNA: For quantitative recovery of low concentrations of RNA (ng/ml), an inert coprecipitant (e.g., glycogen, yeast RNA, or linear acrylamide) should be used.

Peer-Reviewed Papers
15

References

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