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10988537001 Roche

7-Deaza-2′-deoxy-guanosine-5′-triphosphate

Synonym: −N7-dGTP, 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt, 7-Deaza-dGTP

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Properties

InChI Key   DLLXAZJTLIUPAI-UHFFFAOYSA-N
assay   ≤4% (7-Deaza-dGDP, HPLC)
  95% (7-Deaza-dGTP, HPLC)
form   solution
packaging   pkg of 200 μL (10 mM, 2 μmol)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.

Application

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).

Preparation Note

Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.

Analysis Note

Absorption: 25 A260 units of product correspond to 1 mg.

Absorbance determination: A250/A260, A280/A260, A290/A260

Other Notes

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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