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dATP

Lithium salt

Synonym: 2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, dATP, deoxy-adenosine-5′-triphosphate, 2′-

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Properties

Related Categories 100 mM Solutions, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
InChI Key   YJWCICGGRLOGEH-VWZUFWLJSA-M
assay   ≥98%
form   solution
usage   sufficient for 6,250 standard reactions (PCR)
packaging   pkg of 250 μL (25 μmol; 100 mM)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Quality

Typical analysis:
• ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
• Absorbance determination A250/A260, A280/A260, A290/A260
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Unit Definition

1 μmol dATP = 15.0 A260 units

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

dATP has been used to resume transcription.

Biochem/physiol Actions

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

dATP is used in cells for synthesis of DNA by DNA polymerases.

General description

dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s


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Peer-Reviewed Papers
15

References

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