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11074547001 Roche

Insulin-Transferrin-Sodium Selenite Supplement

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Properties

Related Categories Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements,
sterility   non-sterile; 0.2 μm filtered
assay   >98% (Transferrin, SDS-PAGE)
  >99% (Insulin, HPLC)
  >99% (Sodium selenite)
form   lyophilized
packaging   pkg of 50 mg (for 5 l medium)
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)

Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

Application

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.

General description

The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite- synthetic

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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