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11088947001 Roche

BM Condimed H1

Hybridoma Cloning Supplement

Synonym: hybridoma cloning supplement

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Properties

Related Categories Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements,
form   solution
packaging   pkg of 100 mL (10x)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.

Features and Benefits

Contents
Solution in RPMI 1640, filtered through 0.2μm pore size membrane

General description

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.

Physical form

Solution in RPMI 1640, filtered through 0.2 μm pore size membrane

Application

BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

Cell Line Origin

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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