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11117831001 Roche

cDNA Synthesis Kit



Quality Level   100
mfr. no.   Roche
packaging   kit of for up to 10 reactions


General description

cDNA Synthesis System; Instructions For Use

Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA.

The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann.
Sample: 1 - 20 μg total RNA, 0.3 - 2 μg mRNA
Assay time: 60 minutes for 1st strand synthesis, 120 minutes for 2nd strand synthesis
Product: double-stranded cDNA copy of an RNA sample


Optimized one-tube procedure for the synthesis of double-stranded cDNA from total RNA or mRNA.
The ds cDNA can be used for the construction of non-directional cDNA libraries, as a starting point for substractive hybridization experiments to enrich differentially expressed genes, and for the in vitro transcription of whole cDNA populations to generate labeled cRNA for hybridization on DNA microarrays.


• AMV Reverse Transcriptase Buffer, 5x concentrated
• AMV Reverse Transcriptase (25 U/μl)
• 1,4-Dithiothreithol (0.1 M)
• Protector RNase Inhibitor (25 U/μl)
• Oligo (dT)15 primer, 200 μM (1 μg/μl)
• Oligo [(dT24 )T7promotor]65 primer, 100 μM (2 μg/μl)
• dNTP mixture (each nucleotide, 10 mM)
• Control neo mRNA (0.2 μg/μl)
• 2nd strand synthesis buffer, 5x concentrated
• 2nd strand enzyme blend (mixture of DNA Polymerase I, E. coli Ligase and RNase H)
• T4 DNA Polymerase (1 U/μl)
• Double-distilled water
• RNase I (10 U/μl)
• Proteinase K, recombinant, PCR grade (50 U/mll)

Other Notes

Reverse Transcriptases Overview

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

NONH for all modes of transport


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