• USA Home
  • 11170376001 - Anti-Bromodeoxyuridine

EMAIL THIS PAGE TO A FRIEND
11170376001 Roche

Anti-Bromodeoxyuridine

from mouse IgG1 (clone: BMC9318)

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Primary Antibodies
conjugate   unconjugated
clone   BMC9318, monoclonal
biological source   mouse
form   solution
shipped in   dry ice
storage temp.   −20°C
packaging   pkg of 50 μg (500 μl)
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
Quality Level   100
assay   90% (HPLC and SDS-PAGE)
mfr. no.   Roche

Description

General description

Anti-Bromodeoxyuridine is a monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate.

Specificity

The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromo-deoxyuridine does not crossreact with fluorodeoxy-uridine, nor with any endogenous cellular components such as thymidine or uridine.

Immunogen

The epitope is apparently inside the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

Application

Anti-bromodeoxyuridine (Anti-BrdU) antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using:
• Flow cytometry
• Immunohistocytochemistry
• Cryosections
• Paraffin sections

Physical form

Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin.

Quality

The antibody is ≥90% pure as determined by SDS-PAGE with Coomassie-blue staining, and by HPLC.

Specifications

Preparation: BALB/c mice were immunized with a bromodeoxyuridine-bovine serum albumin conjugate. Lymphocytes isolated from the spleen were fused with Ag8.653 myeloma cells to create the BMC 9318 clone. The antibody was produced in ascites in BALB/c mice and purified by ion-exchange chromatography.
No. of tests: 250 (Flow cytometry)

Preparation Note

Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate. Dilutions should be made in PBS (pH 7.4) containing 0.1% BSA to maintain stability of the antibody.

Analysis Note

Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?