11179179001 Roche


from rat tail tendon



Related Categories Attachment Factors, Cell Culture, Reagents and Supplements More...
Quality Level   100
biological source   rat tail
sterility   sterile
form   lyophilized (clear, colorless solution after reconstitution)
packaging   pkg of 30 mg
mfr. no.   Roche
application(s)   cell culture | mammalian: suitable
shipped in   wet ice
storage temp.   2-8°C
Gene Information   rat ... Col1a1(29393)


General description

Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens  are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.


Active on most mammalian cells.


Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.

Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.

Biochem/physiol Actions

Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.

Features and Benefits

Lyophilizate, cell culture grade, 3 vials of 10mg


Biological activity: Tested for the promotion of adherence of HUV-EC cells.

Preparation Note

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.


IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.

Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash


Certificate of Analysis (COA)

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