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11249738001 Roche

Phytohemagglutinin-L (PHA-L)

from Phaseolus vulgaris

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
sterility   non-sterile; 0.2 μm filtered
assay   >95% (SDS-PAGE)
form   lyophilized (clear, colorless solution after reconstitution)
packaging   pkg of 5 mg
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Application

Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.

Biochem/physiol Actions

Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.

General description

PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.

Specifications

Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes

Sequence

PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Same Products - New Packaging
Protocols & Articles
Peer-Reviewed Papers
15

References

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