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11332481001 Sigma-Aldrich

Triton X-100

non-ionic, aqueous solution

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution

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Properties

Related Categories Biochemicals and Reagents, Detergents, Detergents by Applications, Membrane Protein Solubilization
form   solution
mol wt   Mr = 647 (for n=10)
packaging   pkg of 5 × 10 mL
mfr. no.   Roche
application(s)   DNA extraction: suitable
  cell culture | mammalian: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  protein purification: suitable
absorption   = 0.88 at 254 nm at 0.2 % (w/v)
  = 1.11 at 278 nm at 0.5 % (w/v)
shipped in   wet ice
storage temp.   2-8°C
SMILES string   CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI   1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key   HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Octophenolpoly(ethyleneglycolether)n
Especially purified for membrane research

Application

Triton X-100 is used:
• as one of the most commonly used non-ionic aqueous detergents for solubilizing membrane proteins during isolation of membrane-protein complexes
• for native gel electrophoresis
• as a blocking agent in immunocytochemistry
• for permeabilization of slides prior to immunostaining and immunofluorescence
• for permeabilization of slides used for TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) technique

Components

Aqueous solution, 10%(w/v), filled under nitrogen atmosphere in injection vials

Quality

Purity: <2 ppm peroxide content (as H2O2),<200 μM carbonyls,<100 μS conductivity
Note: Purified to reduce levels of unwanted peroxides, carbonyls, metals, and salts. To preserve the low peroxide content of the preparation, always remove aliquots of detergent from the vial with a syringe.

Formula variant

C34H62O11, for n = 10

Preparation Note

Working concentration: 1-5 mM
CMC**: approximately 0.2 mM at +25°C
** CMC: critical micellar concentration

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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