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11371517001 Roche

Swa I

from Staphylococcus warneri

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Properties

Related Categories Molecular Biology, Molecular Biology Enzymes, Restriction Enzymes More...
Quality Level   100
form   solution
packaging   pkg of 200 U (10 U/μl)
mfr. no.   Roche
parameter   25 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.

Application

Swa I is very useful for digesting GC-rich bacterial genomes.

DNA Profile

Number of cleavage sites on different DNAs
• λ: 0
• φX174: 0
• Ad2: 1
• M13mp7: 1
• pBR322: 0
• pBR328: 0
• SV40: 1

Unit Definition

One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.

Analysis Note

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
• A: 0-10%
• B: 10-25%
• H: 100%
• L: 0-10%
• M: 0-10%

Activity in PCR buffer: Not tested

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

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Enzyme Solution    
SuRE/Cut Buffer H 10x concentrated    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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