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11418165001 Roche

Biotin Protein Labeling Kit

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Properties

Related Categories Antibodies, Biotin Labeling Reagents, Biotin/Avidin, Supplementary Products
Quality Level   100
usage   sufficient for 5 labeling reactions
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Packaging

1 kit containing 5 components.

Quality

Function tested.

Preparation Note

Working solution: Preparation of the solutions
• Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
• PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
• Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

Add to Cart

Blocking Reagent (green screw cap)    
Phosphate Buffered Saline (PBS) (blue screw cap)    
D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)    
Dimethylsulfoxide (DMSO) (red screw cap)    
Sephadex G-25 Columns    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Alkaline Phosphatase Protocol & Troubleshooting

When a given nucleic acid is not dephosphorylated using the recommended reaction conditions, try using a higher Mg2+ concentration and spermidine. For 5 pmol of 5′-phosphate termini, dissolve the nuc...
Keywords: Condensations, Precipitation

Biotin Protein Labeling Kit Protocol & Troubleshooting

Alkaline conditions (pH 10-10.5) Conjugation at pH 10 - 10.5 has not been tested. At higher pH, hydrolysis of the ester could occur, and a pH shift to 10-11 will disrupt the ester.
Keywords: Bacterial conjugations, Chromatography, Dialysis, Purification

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Peer-Reviewed Papers
15

References

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