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11420470001 Roche

dUTP

Lithium salt

Synonym: 2′-deoxy-uridine-5′-triphosphate, deoxy-uridine-5′-triphosphate, 2′-

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Properties

Related Categories Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides), dUTP and dUTP Mixes
assay   ≥98%
form   solution
usage   sufficient for 6,250 standard reactions (PCR)
packaging   pkg of 250 μL (25 μmol; 100 mM)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Application

DNA that contains dUTP is susceptible to hydrolysis with Uracil-DNA Glycosylase. Therefore, dUTP is most commonly used (e.g., in PCRs and RT-PCRs with Taq DNA Polymerase) to prevent carryover contamination.However, dUTP is also a substrate for other DNA synthesizing enzymes (e.g., the Kornberg DNA polymerase and the Klenow Enzyme). Therefore, dUTP can be used for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

General description

dUTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).

Contents
dUTP solution in water (pH 7), 100 mM, lithium salt

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Typical analysis:
• ≥98% dUTP (HPLC), =1.5% dUDP (HPLC), =0.5% dUMP (HPLC)
• Absorbance determination A250/A260, A280/A260, A290/A260

Unit Definition

1 μmol dUTP = 9.9 A260 units

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

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