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11585029001 Roche

NBT

4-Nitro blue tetrazolium chloride, crystals

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, Nitrotetrazolium Blue chloride

  • CAS Number 298-83-9

  • Linear Formula C40H30N10O6 · 2Cl

  • Molecular Weight 817.64

  •  Beilstein/REAXYS Number 4115923

  •  MDL number MFCD00012159

  •  PubChem Substance ID 329749491

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Properties

Related Categories Colorimetric, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization
Quality Level   100
packaging   pkg of 5 g
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C
SMILES string   [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Application

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.

Quality

Performance-controlled in the LDH assay.

Preparation Note

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Analysis Note

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

NBT Protocol

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), for instance, FastGreen FCF or Nuclear Fast Red. For details, see the protocol described in the Roche ...
Keywords: In Situ hybridization, Nucleic acid hybridization

Peer-Reviewed Papers
15

References

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