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11585045001 Roche

Cellular DNA Fragmentation ELISA

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Properties

Quality Level   100
usage   sufficient for ≤500 tests
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The Cellular DNA Fragmentation ELISA is a photometric enzyme-linked immunosorbent assay (ELISA) for the detection of 5’-bromo-2’-deoxy-uridine (BrdU)-labeled DNA fragments in culture supernatants and cell lysates. BrdU is used as a metabolic labeling agent by the nuclear DNA of target cells. This BrdU-labeled DNA can be detected easily and quantified using a monoclonal antibody against BrdU in an ELISA. This kit is a non-radioactive alternative to the [3H]-thymidine release assay, the [51Cr]-release assay, and the [3H]-thymidine DNA fragmentation assay.

Application

For research use only. Not for use in diagnostic procedures.
The Cellular DNA Fragmentation ELISA is for the quantification of BrdU-labeled DNA fragments either released from cells during necrosis or cell-mediated cytotoxicity, or within the cytoplasm of apoptotic cells.

Features and Benefits

• Nonradioactive assay system
• Sensitive (1 x 103 cells/well)
• Low assay background
• No additional dilution steps required
• No washing of the cells required
• Results obtained correlate to those obtained by standard methods
• Denaturation/fixation of DNA in the microplate by microwave irradiation
• Fast (4.5 - 5.5 hours)

Packaging

1 kit containing 8 components.

Preparation Note

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Storage conditions (working solution): Storage of coated plates: 1 week at 2 to 8 °C.

The anti-BrdU-POD stock solution is stable at 2 to 8 °C for several months; for long- term storage it is recommended to store the solution in aliquots at -15 to -25 °C;
The anti-BrdU-working solution should not be stored.
For the anti-DNA-clone MCA-33 we do no have stability data. However it should be no problem to aliquot and store this non-conjugated antibody at -15 to -25 °C for longer storage.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

Add to Cart

Anti-DNA Antibody (clone MCA-33)    
Anti-BrdU Peroxidase antibody, Fab Fragments (clone BMG 6H8)    
Coating Buffer    
Washing Buffer    
Incubation Buffer    
Substrate Solution    
BrdU Labeling Reagent    
Adhesive Cover Foils    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Cellular Apoptosis Assays | Programmed Cell Death

Apoptosis, or programmed cell death, is a growth-limiting regulatory mechanism by which cells can trigger their own death in response to extracellular signals because of irreparable cellular or DNA d...
Keywords: Apoptosis, Autophagy, Cancer, Cell culture, Detection methods, Diseases, Enzyme-linked immunosorbent assay, Flow cytometry, Immunofluorescence, Immunohistochemistry, Microscopy, Phosphorylations

Peer-Reviewed Papers
15

References

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