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11585614910 Roche

DIG-High Prime DNA Labeling and Detection Starter Kit II

Green Alternative

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DIG System Kits, Greener Alternative Products, Greener Life Science Products,
Quality Level   100
usage   sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
  sufficient for 24 blots (blots of 100 cm2)
mfr. no.   Roche
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
shipped in   dry ice
storage temp.   −20°C

Description

General description

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:
• in Southern blots
• in northern blots
• in dot blots
• in colony and plaque hybridizations
• for all types of filter hybridization
• for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Packaging

1 kit containing 7 components.

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

Add to Cart

DIG-High Prime 5x concentrated    
DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml    
DNA Dilution Buffer    
Anti-Digoxigenin-AP Conjugate antibody    
CSPD ready-to-use    
Blocking Solution 10x concentrated    
DIG Easy Hyb Granules    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Digoxigenin (DIG) Methods and Kits

The DIG System is the nonradioactive technology of choice to label and detect nucleic acids for multiple applications. The system is based on a steroid isolated from digitalis plants (Digitalis purpu...
Keywords: Amplification, Cloning, Diagnostic, Electrophoresis, Enzyme-linked immunosorbent assay, Gas chromatography, Gel electrophoresis, Immobilization, In Situ hybridization, Northern blot, Nucleic acid hybridization, Polymerase chain reaction, Purification, Transcription

Peer-Reviewed Papers
15

References

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