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11636138001 Roche

PCR Optimization Kit

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Properties

Related Categories Molecular Biology, PCR Enhancers, PCR/Amplification More...
usage   sufficient for ≤100 assays (one-step)
  sufficient for ≤50 assays (two-step)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

The PCR Optimization Kit is the convenient and reliable solution for optimizing PCR experiments. It contains a set of buffers with different pH and magnesium concentrations spanning the most often-used reaction conditions. Four different additives allow additional optimization to increase yield and specificity.
The optimal reaction conditions of a PCR experiment depend on the template DNA and the respective primer pair. The main parameters that may vary between different applications are the magnesium concentration, pH of the reaction buffer, and the temperature and time of the different cycling steps. Additionally, various additives may increase the specificity and yield of some applications.
Therefore, it is generally recommended to optimize PCR conditions for each new template/primer pair. However, the preparation of the various solutions is time-consuming and may increase the risk of contamination. This is where the PCR Optimization Kit can help in optimization.

Application

With the ready-to-use solutions supplied in the PCR Optimization Kit the best PCR conditions can be determined by easily varying MgCl2 concentration, the pH of the buffer, and additives like DMSO or glycerol. DMSO, for instance, is reported to reduce nonspecific priming, while gelatin and glycerol stabilize Taq DNA polymerase during PCR, which generally increases the yield. The ready-to-use solutions save time for your research and minimize the risk of contamination. In combination with Roche′s FastStart High Fidelity PCR System this kit allows you to define optimal conditions for multiplex PCR experiments with astonishing results.

Packaging

1 kit containing 6 components.

Quality

Each lot of the PCR Optimization Kit is function tested in PCR using human genomic DNA for the absence of contaminating DNA, and exo- or endonuclease activity according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit component only

Description

Product #

Add to Cart

PCR Optimization Buffers, 100 mM Tris-HCl, labeled A-P 500 mM KCl, 10-25 mM MgCl2 10x concentrated    
DMSO    
Gelatin Stock Solution, in water 1.0% (w/v)    
(NH4)2SO4 Stock Solution 500 mM    
Glycerol Stock Solution, in water 50% (w/v)    
MgCl2 Stock Solution 25 mM    
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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