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11644793001 Roche

Cytotoxicity Detection Kit (LDH)

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Properties

Related Categories Cell Analysis, Cell Biology More...
Quality Level   100
usage   sufficient for ≤2,000 tests
packaging   pkg of 1 kit
mfr. no.   Roche
application(s)   cell analysis: suitable
  detection: suitable
  protein quantification: suitable
λmax   490 nm
detection method   colorimetric
shipped in   dry ice
storage temp.   −20°C

Description

General description

A nonradioactive alternative to the [51Cr] and [3H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.

Application

The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:
• Detection and quantification of cell-mediated cytotoxicity
• Determination of mediator-induced cytolysis
• Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries
• Determination of cell death in bioreactors
• Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Packaging

1 kit containing 2 components.

Preparation Note

Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.

Vial 2 Dye solution:
Ready-to-use solution.

Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.

Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

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Catalyst (Diaphorase/NAD+ mixture)    
Dye Solution (INT and sodium lactate)    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Request a Serum Sample
Protocols & Articles

Protocols

Cytotoxicity Detection Kit (LDH) Protocol & Troubleshooting

LDH is reported to be stable for one week at room temperature, and for three weeks at +2 to +8 °C; freezing should be avoided.
Keywords: Phase transitions

Peer-Reviewed Papers
15

References

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Description

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91963 Cytotoxicity detection kit, 100 tests

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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